Biochemical analysis of NSs from different tospoviruses
نویسندگان
چکیده
منابع مشابه
Identification of Common Epitopes on a Conserved Region of NSs Proteins Among Tospoviruses of Watermelon silver mottle virus Serogroup.
ABSTRACT The NSs protein of Watermelon silver mottle virus (WSMoV) was expressed by a Zucchini yellow mosaic virus (ZYMV) vector in squash. The expressed NSs protein with a histidine tag and an additional NIa protease cleavage sequence was isolated by Ni(2+)-NTA resins as a free-form protein and further eluted after sodium dodecyl sulfate-polyacrylamide gel electrophoresis for production of rab...
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Tospoviruses belong to the sole phytovirus genus, Tospovirus, in the family Bunyaviridae. Tospoviruses are known to be exclusively transmitted by thrips belonging to the family Thripidae and subfamily Thripinae. Of the known 1,710 species of Thripidae only 14 thrips species are currently reported to transmit tospoviruses. Thrips-transmitted tospoviruses cause severe yield losses to several econ...
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Recent studies analyzing nucleocapsid protein gene have revealed the existence of a broad genetic diversity of tospoviruses. In Brazil, six tospoviruses have been reported in agricultural, horticultural and floral crops. The viruses Tomato spotted wilt virus (TSWV), Tomato chlorotic spot virus (TCSV), Groundnut ringspot virus (GRSV) and Chrysanthemum stem necrosis virus (CSNV) are well-known pa...
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ژورنال
عنوان ژورنال: Virus Research
سال: 2017
ISSN: 0168-1702
DOI: 10.1016/j.virusres.2017.09.020